91丝瓜视频

Seminar Title:

Fluorescence microscopy: a minimum for maximal spatio-temporal resolution

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Abstract:

I will discuss MINFLUX, a molecular localization and superresolution method that has reached Angstr枚m localization precision and resolution of the size of a fluorescent molecule. MINFLUX and the related MINSTED concept are being established for routine applications in cell and molecular biology, and ultimately structural biology. Relying on much fewer fluorescence photons than the widely used camera-based localization methods, these localization techniques are poised to characterize dynamic processes of single proteins, as demonstrated by tracking the nanometer conformational changes of the motor proteins kinesin-1 [1,2] and dynein in living cells [3]. MINFLUX has been demonstrated to measure intramolecular distances with Angstr枚m precision, providing a precise and reliable alternative to FRET that also gives absolute 3D coordinates and hence vectorial 3D distances [4]. Harnessing confocal detection, MINFLUX provides nanometerrange resolution deeper down in layers of cells and mildly scattering tissues [5]. Finally, I will show an arguably surprising ability of MINFLUX to separate individual identical fluorophores without sequential ON/OFF switching or activation of fluorescence [6]. Thus, the simultaneous, uninterrupted, nanometer-scale tracking [7] and imaging of multiple, identical (same-color) fluorophores becomes possible for the first time. This novel superresolution principle should allow MINFLUX to reveal the conformational changes of individual biomolecules in their native environment.

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Bio:

Stefan Hell is a director at both the Max Planck Institute for Multidisciplinary Sciences in G枚ttingen and the Max Planck Institute for Medical Research in Heidelberg, Germany.

Hell is credited with having conceived, validated and applied the first viable concept for overcoming Abbe鈥檚 diffraction-limited resolution barrier in a light-focusing fluorescence microscope. For this accomplishment he has received numerous awards, including the 2014 Kavli Prize in Nanoscience and the Nobel Prize in Chemistry.

Stefan Hell received his doctorate (1990) in physics from the University of Heidelberg. From 1991 to 1993 he worked at the European Molecular Biology Laboratory, followed by stays as a senior researcher at the University of Turku, Finland, between 1993 and 1996, and as a visiting scientist at the University of Oxford, England, in 1994. In 1997 he was appointed to the MPI for Biophysical Chemistry (now MPI for Multidisciplinary Sciences) in G枚ttingen as a group leader, and was promoted to director in 2002.