BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//132.216.98.100//NONSGML kigkonsult.se iCalcreator 2.20.4//
BEGIN:VEVENT
UID:20260807T111422EDT-2600edoNfU@132.216.98.100
DTSTAMP:20260807T151422Z
DESCRIPTION:Dr. Anne-Claude Gingras\nAssistant Professor\nDepartment of Mol
 ecular Genetics\nUniversity of Toronto\n“Charting kinase and phosphatase i
 nteractomes: From interaction\nproteomics to functional insights”\nAnne-Cl
 aude Gingras received her Ph.D. from 91˿����Ƶ\nUniversity in 2001 for her st
 udies on translational control\,\nperformed under the guidance of Nahum So
 nenberg.  She then\njoined the laboratory of Ruedi Aebersold at the Instit
 ute for\nSystems Biology in Seattle\, where she developed proteomics\nappr
 oaches to study protein-protein interactions.  She\nestablished her own la
 boratory in 2005 at the Samuel Lunenfeld\nResearch Institute at Mount Sina
 i Hospital in Toronto\, where she\ncurrently holds a Canada Research Chair
  in Functional Proteomics\nand the Lea Reichmann Chair in Cancer Proteomic
 s.  She is an\nAssistant Professor in the Department of Molecular Genetics
  of the\nUniversity of Toronto. Her group focuses on the identification an
 d\nfunctional characterization of protein interaction modules\nsurrounding
  serine/threonine phosphatases. Active areas of research\ninclude understa
 nding how the PP4 phosphatase modulates gene\nexpression via regulation of
  transcription elongation\, mRNA\ncapping\, and splicing. The Gingras lab 
 is also investigating the\nfunction of a novel protein complex that they h
 ave identified\nsurrounding the protein CCM3\, whose gene is mutated in fa
 milial\ncases of angioma.  Anne-Claude Gingras is also involved in the\nde
 velopment of robust approaches for the analysis of protein\ninteraction ne
 tworks in yeast and human and for the study of\nenzyme-substrate relations
 hips within the interaction\nnetworks.\n \nProtein phosphorylation mediate
 s cellular responses to growth\nfactors\, environmental signals\, and inte
 rnal processes by the\nregulation of protein interactions\, enzyme activit
 y or protein\nlocalization. However\, the protein interactions of kinases\
 ,\nphosphatases\, their regulatory subunits and substrates remain\nsparsel
 y mapped. Two interaction mapping projects will be\npresented: 1) To chart
  the budding yeast kinase and phosphatase\ninteraction (KPI) network\, we 
 systematically characterized protein\nkinase and phosphatase complexes by 
 sensitive affinity purification\ncoupled to mass spectrometric identificat
 ion (AP-MS).  We\nidentified a KPI network of 1\,844 interactions that con
 tained many\ndense local regions.  Notably\, the cell cycle phosphatase\nC
 dc14 associated with multiple kinases that revealed roles for\nCdc14 in mi
 togen-activated protein kinase signaling\, the DNA damage\nresponse and me
 tabolism\, while interactions of the target of\nrapamycin complex 1 (TORC1
 ) uncovered new effector kinases in\nnitrogen and carbon metabolism. An ex
 tensive backbone of\nkinase-kinase interactions cross-connects the proteom
 e and may\nserve to coordinate diverse cellular responses. 2) To identify
 \npotential new substrates\, regulators and targeting subunits for the\nhu
 man PP2A phosphatase\, we performed an iterative AP-MS\napproach.  This al
 lowed us to uncover an interaction network\ncontaining 365 proteins.  With
 in this network\, we further\nfocused on the characterization of the large
 st stable PP2A\ncontaining complex identified to date.  This complex\, cal
 led\nSTRIPAK\, is evolutionarily conserved and implicated in cytoskeletal
 \nevents.  A core component of STRIPAK\, CCM3\, is mutated in\nfamilial ca
 ses of cerebral cavernous malformations\, which are\nvascular anomalies of
  the brain capillaries\, and our recent results\nindicate that STRIPAK is 
 involved in the disease.  These two\nprojects will be presented within the
  context of the development of\nbioinformatics and statistical analysis to
 ols for protein-protein\ninteractions.\n \n \nGeneral review of interactio
 n proteomics:\nGingras et al.\, Nat Review Mol Cell Biol\, 2007\, 8:645\n 
 \nProtocols for affinity purification coupled to mass\nspectrometry\nChen 
 and Gingras\, Methods\, 2007\, 42-298\n \nThe scientific presentation will
  focus on unpublished data\nand:\n1) Goudreault et al.\, Mol Cell Proteomi
 cs\, 2009\, 8:157\n2) Breitkreutz et al.\, Science\, 2010 (in press)\n3) C
 hoi et al.\, Mol Sys Biol\, 2010 (in press)\n
DTSTART:20100601T140000Z
DTEND:20100601T140000Z
LOCATION:Life Sciences Complex\, CA\, QC\, Montreal\, H3G 0B1\, 3649 promen
 ade Sir William Osler
SUMMARY:Biochemistry Seminar Dr. Anne-Claude Gingras
URL:/channels/event/biochemistry-seminar-dr-anne-claud
 e-gingras-163732
END:VEVENT
END:VCALENDAR