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DTSTAMP:20260807T151422Z
DESCRIPTION:Dr Jack D. Keene\nJames B. Duke Professor\nMolecular Genetics &
  Microbiology\nDuke University Medical Center\n“Coordinated Dynamics of RN
 A Regulation by RNA-Binding\nProteins”\nWhile RNA-binding proteins (RBPs) 
 coordinate many key decisions\nduring cell growth and differentiation\, th
 e dynamics of RNA\nstability and translation that depend upon RBPs have no
 t been\nstudied on a global basis. Recent findings demonstrate that\nmulti
 ple mRNAs are co-regulated by sequence-specific RBPs that\norchestrate the
 ir splicing\, export\, stability\, localization and\ntranslation. These ob
 servations have given rise to a model in which\nmRNAs that encode function
 ally related proteins are coordinated\ndynamically during cell growth and 
 differentiation as\npost-transcriptional RNA operons or regulons. I will r
 eview\nrecently discovered examples of post-transcriptional RNA operons in
 \nmammalian cells\, trypanosomes and budding yeast\, and their\npotential 
 importance in processes such as immune response\,\noxidative stress and di
 sease. I will demonstrate how quantitative\nRNA dynamics involving the HuR
  RBP can be assessed\nprobabilistically using the RIP-chip method to deriv
 e a\nquantitative phenotype from the drug-gene-phenotype Connectivity\nMap
  that allows us to identify small molecule drugs affecting\nposttranscript
 ional regulatory networks during T-cell activation.\nIn addition\, I will 
 discuss the use of the novel PAR-CLIP approach\nof Tuschl and coworkers to
  identify precise HuR RBP binding sites\nand how those data can complement
  RIP-chip data to visualize\ncoordination of global RNA dynamics affecting
  distinct functional\noutcomes.\nMorris\, A. et al. (2008) Ribonomic analy
 sis of human Pum1\nreveals cis-trans conservation across species despite e
 volution of\ndiverse mRNA target sets. Molecular and Cellular Biology 28:
 \n4093-4103.\nMukherjee\, N. et al. (2009) Coordinated post-transcriptiona
 l mRNA\npopulation dynamics during T-cell activation. Molecular Systems\nB
 iology 5: 288.\nMorris\, A.D. et al. (2009) Systematic analysis of\nposttr
 anscriptional gene expression. Wiley Interdisciplinary\nReviews\, WIRES: S
 ystems Biology and Medicine.\n[doi:10.1002/wsbm.54]\nHafner\, M. et al. (2
 010) Transcriptome-wide identification of\nRNA-binding protein and microRN
 A binding sites by PAR-CLIP. Cell\n141: 129-141.\n
DTSTART:20100525T160000Z
DTEND:20100525T160000Z
LOCATION:McIntyre Medical Building\, CA\, QC\, Montreal\, H3G 1Y6\, 3655 pr
 omenade Sir William Osler
SUMMARY:Biochemistry Seminar Dr. Jack Keene
URL:/channels/event/biochemistry-seminar-dr-jack-keene
 -163567
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