BEGIN:VCALENDAR VERSION:2.0 PRODID:-//132.216.98.100//NONSGML kigkonsult.se iCalcreator 2.20.4// BEGIN:VEVENT UID:20260728T152759EDT-78292bf6Em@132.216.98.100 DTSTAMP:20260728T192759Z DESCRIPTION:Dr. Lawrence Kleiman\nProfessor\nDepartments of Medicine & Micr obiology and Immunology -\n91Ë¿¹ÏÊÓƵ\nLady Davis Institute for Medical Resear ch\nJewish General Hospital\n'The Regulation of tRNALys3 annealing to HIV- 1 genomic RNA'\nDuring HIV-1 assembly\, tRNALys3 anneals to viral RNA\, an d upon\nnew viral infection\, serves as the primer for the initiation of\n reverse transcription of viral RNA into proviral DNA. Regulation of\ntRNAL ys3 annealing during viral assembly involves several factors:\n1) A select ive concentration of tRNALys3 at the cellular site of\nviral assembly\, pr omoted by a specific interaction between viral\nGag and lysyl-tRNA synthet ase. 2) A configura-tion of viral Gag and\nGagPol that promotes the transf er of tRNALys3 from lysyl tRNA\nsynthetase to reverse transcriptase to vir al RNA. 3) Viral and host\ncell factors that facilitate RNA annealing\, su ch as viral\nnucleocapsid sequences and RNA helicase A. tRNALys3 annealing \nappears to be a multi-step process\, where the initial stage is\ncarried out by viral Gag before viral budding and viral protein\nprocessing\, and a final stage consisting of the re-annealing of\nGag-annealed tRNALys3 by mature nucleocapsid\, NCp7.\n \n \nReferences:\n \nKleiman\,L. Jones\, C. \, and Musier-Forsyth\, K. Formation of the\ntRNALys Packaging Complex in HIV-1 (2009) FEBS Lett. 584:\n359-365\n \nGuo\, F.\, Saadatmand\, J.\, Niu \, M.\, and Kleiman\, L. Roles of Gag\nand NCp7 in facilitating tRNALys3 a nnealing to viral RNA in HIV-1.\n(2009) J. Virol. 83: 8099-8107\n \nHalwan i\,  R.\, Cen\, S.\,  Javanbakht\,  H.\,\nSaadatmand\,  J.\, Kim\, S.\, Sh iba\, K.\, and Kleiman\,L. Cellular\ndistribution of lysyl-tRNA synthetase and its interaction with Gag\nduring HIV-1 assembly. (2004) J. Virol. 78: 7553-7564\n \nJavanbakht\, H.\, Halwani\, R.\, Cen\, S.\, Sadaatmand\, J. \,\nMusier-Forsyth\, K.\, Gottlinger\, H.\, and Kleiman\, L. The interacti on\nbetween HIV-1 Gag and human lysyl-tRNA synthetase during viral\nassemb ly. (2003) J. Biol. Chem. 278: 27644-27651\n DTSTART:20100426T160000Z DTEND:20100426T160000Z LOCATION:McIntyre Medical Building\, CA\, QC\, Montreal\, H3G 1Y6\, 3655 pr omenade Sir William Osler SUMMARY:Biochemistry Seminar URL:/channels/event/biochemistry-seminar-116030 END:VEVENT END:VCALENDAR