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DESCRIPTION:Mechanisms of load adaptation by endocytic actin networks\n\nMa
 tthew Akamatsu\, University of Washington\n	Tuesday March 24\, 12-1pm\n	Zoom
  Link: https://mcgill.zoom.us/j/87078928687\n	In Person: 550 Sherbrooke\, R
 oom 189\n	\n	Abstract: Self-organization is a hallmark of biological process
 es wherein local (neighboring) interactions constrained by the geometrical
  environment and physical laws give rise to emergent architectures and ada
 ptive behaviors. In clathrin-mediated endocytosis\, branched actin cytoske
 letal networks deform the cell’s plasma membrane against tension to intern
 alize cargo. Characteristic of self-organizing systems\, these networks ad
 apt their size and architecture under high load to increase force-producin
 g capability. However\, the mechanisms that govern this mechano-adaptation
  remain poorly understood. Our lab combines biophysical modeling\, quantit
 ative microscopy of gene-edited human stem cells\, and high-resolution ima
 ge analysis to investigate cytoskeletal self-organization and load adaptat
 ion in mammalian endocytosis.\n\nA minimal biophysical model recapitulated
  the mechano-adaptive behavior of these endocytic cytoskeletal networks. S
 imulations predicted that under elevated load\, endocytic actin networks r
 espond by nucleating actin filaments and shifting their orientations to a 
 more load-bearing arrangement. To test this prediction\, we used quantitat
 ive lattice light-sheet microscopy of gene-edited human induced pluripoten
 t stem cells and transient osmotic shocks to increase membrane tension. In
 deed\, elevating membrane tension increased branched actin assembly and th
 e fraction of endocytic sites assembling an actin cytoskeleton\, specifica
 lly at the basal cellular surface. With simulations\, we identified severa
 l putative factors that contribute to load adaptation\, including the tran
 sient stalling of endocytic sites and force-dependent association rates be
 tween capping protein and actin filaments. These adaptive behaviors allow 
 a dynamic actin cytoskeleton to robustly and reliably deform cellular memb
 ranes in the stochastic cellular environment.\n
DTSTART:20260324T160000Z
DTEND:20260324T170000Z
SUMMARY:QLS Seminar Series - Matthew Akamatsu 
URL:/arts/channels/event/qls-seminar-series-matthew-ak
 amatsu-371875
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